Polyhydroxyalkanoates (PHA)

B. Upstream Process

Upstream
Schematic representation of the fill-and-draw fed-batch mode of fermentation system: 1) Substrate reservoir; 2) 0.2 μM microbial filter; 3) Peristaltic pump; 4) Two port adaptor; 5) Culture vessel; 6) Air condenser; 7) Heating jacket; 8) Pinch clamp; 9) Baffle: 10) Propeller shaft; 11) Antifoam breaker; 12) Propeller; 13) Air sparger; 14) Vessel sampling tube; 15) Sampling unit system; 16) Syringe; 17) 50 mL, centrifuge tube; 18) Plastic tag; 19) Foam reservoir; 20) Air flow meter; 21) Air pump. (Biglari et. al. 2020)

References

  1. Biglari, N., Orita, I., Fukui, T. and Sudesh, K. (2020) A study on the effects of increment and decrement repeated fed-batch feeding of glucose on the production of poly(3-hydroxybutyrate) [P(3HB)] by a newly engineered Cupriavidus necator NSDG-GG mutant in batch fill-and-draw fermentation. Journal of Biotechnology, 307, pp.77-86.
  2. Biglari, N., Ganjali Dashti, M., Abdeshahian, P., Orita, I., Fukui, T. and Sudesh, K. (2018) Enhancement of bioplastic polyhydroxybutyrate P(3HB) production from glucose by newly engineered strain Cupriavidus necator NSDG-GG using response surface methodology. 3 Biotech, 8(8).
PHAb

Ecobiomaterial Laboratory

Lab 409, G09A
School of Biological Sciences
Universiti Sains Malaysia
11800 USM Penang, Malaysia

E-mail: ksudesh@usm.my

Ecobiomaterial Laboratory

Lab 409, G09A
School of Biological Sciences
Universiti Sains Malaysia
11800 USM Penang, Malaysia

E-mail: ksudesh@usm.my

PHAb
PHAb
PHAb