Bacteria synthesis and store PHAs when they lack the complete range of nutrients required for cell division but have generous supply of carbon. Quantity of PHA accumulated increased as the carbon to nitrogen ratio increased [3]. The biosynthesis of PHA was shown to be initiated by magnesium or sulphate deficiency, as well as a nitrogen, phosphate [4, 5] and/or oxygen limitation [6].
Cell devisions of Ralstonia eutropha H16
Ralstonia eutropha H16 grown under high nitrogen molarity

Ralstonia eutropha H16 grown in mineral medium supplemented with 50 mM urea as nitrogen source
Ralstonia eutropha H16 grown in mineral medium supplemented with 5 mM urea as nitrogen source
